Reverse Transcriptase Having Improved Thermostability

The present invention relates to a reverse transcriptase having improved thermal stability. More specifically, the present invention relates to a mutant transcriptase having improved thermal stability, in which one or more amino acids selected from the group consisting of 63rd glutamine (Q63), 264th...

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Bibliographische Detailangaben
Hauptverfasser:	HWANG, BYOUNG OH, PARK, HAN OH, JOO, SUNG MO, YANG, SUNG JUN
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a reverse transcriptase having improved thermal stability. More specifically, the present invention relates to a mutant transcriptase having improved thermal stability, in which one or more amino acids selected from the group consisting of 63rd glutamine (Q63), 264th lysine (K264), 295th lysine (K295), 306th threonine (T306), 346th glutamic acid (E346), 408th proline (P408), 438th histidine (H438), and 454th asparagine (N454) of an amino acid sequence of an M-MLV-derived transcriptase represented by SEQ ID NO: 1 are substituted by another amino acid. The mutant transcriptase of the present invention shows excellent thermal stability compared to a wild-type transcriptase. Even when a transcription product is not effectively obtained due to a structural disturbance of specific RNA capable of forming a stable secondary structure even at a high temperature, a transcription activity is stably shown, and desired cDNA can be obtained. 본 발명은 열안정성이 증가된 역전사효소에 관한 것으로서, 보다 상세하게는 서열번호 1로 기재되는 M-MLV 유래 역전사효소의 아미노산 서열의 63번째의 글루타민(Q63), 264번째의 리신(K264), 295번째의 리신(K295), 306번째의 트레오닌(T306), 346번째의 글루탐산(E346), 408번째의 프롤린(P408), 438번째의 히스티딘(H438) 및 454번째의 아스파라긴(N454)으로 이루어진 군으로부터 선택되는 하나 이상의 아미노산이 다른 아미노산으로 치환되어 열안정성이 증가된 돌연변이 역전사효소에 관한 것이다. 본 발명의 돌연변이 역전사효소는 야생형 역전사효소와 비교하여 뛰어난 열안정성을 나타내므로, 고온에서도 안정한 2차 구조를 형성할 수 있는 특정한 RNA의 구조적 방해로 인해 효율적으로 역전사 산물을 얻지 못하는 경우라도 안정적으로 역전사 활성을 나타내어 원하는 cDNA를 얻을 수 있다는 장점이 있다.