Primer set method and kit for selecting Canola events

The present invention relates to a primer set, a method, and a kit capable sorting five gene living modified canola events (Topas 19/2, Rf3, Ms8, RT73, and T45-) approved to be imported and circulated from a fluid event by using multiplex polymerase chain reaction (M-PCR). The present invention prov...

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Bibliographische Detailangaben
Hauptverfasser:	CHOI, WON KYUN, MOON, JEONG CHAN, SHIN, SU YOUNG, KIM, IL RYONG, SEOL, MIN A, LEE, JUNG RO, EUM, SOON JAE, JO, BEOM HO, SONG, HAE RYONG
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a primer set, a method, and a kit capable sorting five gene living modified canola events (Topas 19/2, Rf3, Ms8, RT73, and T45-) approved to be imported and circulated from a fluid event by using multiplex polymerase chain reaction (M-PCR). The present invention provides a primer set, a method, and a kit for simultaneously sorting five gene modified canola events through M-PCR by using a PCR primer for detecting the gene modified canola events (Topas 19/2, Rf3, Ms8, RT73, and T45). The method and kit of the present invention can be useful in accurately determining gene modified plants. 본 발명은 다중 중합효소연쇄반응(Multiplex-Polymerase Chain Reaction; M-PCR)을 이용하여 유체 이벤트를, 보다 상세하게는 대한민국 내로 수입이 승인되어 유통되는 5종의 유전자 변형 캐놀라(Living modified canola) 이벤트-Topas 19/2, Rf3, Ms8, RT73, 및 T45-를 선별할 수 있는 프라이머 세트, 방법 및 키트에 관한 것이다. 본 발명은 상기 유전자 변형 캐놀라 이벤트-Topas 19/2, Rf3, Ms8, RT73, 및 T45 검출용 PCR 프라이머를 이용하여 다중 중합효소연쇄반응으로 5종의 유전자 변형 캐놀라 이벤트를 동시에 선별하는 프라이머 세트. 방법 및 키트를 제공한다. 본 발명의 선별방법 및 키트는 유전자 변형 작물의 정확한 판정을 위하여 유용하게 사용될 수 있다.