Method for increasing lipid productivity and growth of microalgae by using an overexpression of bHLH transcription factors
The present invention relates to a recombinant vector, pNs302(bHLH2) which overexpresses a gene (bHLH2) encoding basic helix-loop-helix transcription factor, transgenic microalgae in which the recombinant vector, pNs302(bHLH2), is inserted, and a method for enhancing lipid productivity and a growth...
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| creator | KWON, SO HEE JEONG, BYEONG RYOOL LEE, BONG SOO JEON, SEUNG JIB PARK MIN SUNG CHOI, GANG GUK YANG, JI WON KANG, NAM KYU |
| description | The present invention relates to a recombinant vector, pNs302(bHLH2) which overexpresses a gene (bHLH2) encoding basic helix-loop-helix transcription factor, transgenic microalgae in which the recombinant vector, pNs302(bHLH2), is inserted, and a method for enhancing lipid productivity and a growth rate of microalgae by culturing the microalgae in a nitrogen-depleted medium and/or an osmosis stress medium. The recombinant vector, pNs302(bHLH2), contains a gene (bHLH2) encoding the basic helix-loop-helix transcription factor, tubulin promoter (P_tub) and tubulin terminator (T_tub).
본 발명은 염기성 나선 루프 나선 전사 인자 단백질을 코딩하는 유전자(bHLH2)를 과발현시키는 재조합 벡터 pNs302(bHLH2), 상기 bHLH2(bHLH2)가 삽입되어 있는 형질전환 미세조류 및 상기 미세조류를 질소결핍 배지 및/또는 삼투압 스트레스 배지에서 배양하여 미세조류의 지질 생산성 및 생장률을 증가시키는 방법에 관한 것이다. |
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본 발명은 염기성 나선 루프 나선 전사 인자 단백질을 코딩하는 유전자(bHLH2)를 과발현시키는 재조합 벡터 pNs302(bHLH2), 상기 bHLH2(bHLH2)가 삽입되어 있는 형질전환 미세조류 및 상기 미세조류를 질소결핍 배지 및/또는 삼투압 스트레스 배지에서 배양하여 미세조류의 지질 생산성 및 생장률을 증가시키는 방법에 관한 것이다.</description><language>eng ; kor</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2016</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161212&DB=EPODOC&CC=KR&NR=20160142024A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20161212&DB=EPODOC&CC=KR&NR=20160142024A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>KWON, SO HEE</creatorcontrib><creatorcontrib>JEONG, BYEONG RYOOL</creatorcontrib><creatorcontrib>LEE, BONG SOO</creatorcontrib><creatorcontrib>JEON, SEUNG JIB</creatorcontrib><creatorcontrib>PARK MIN SUNG</creatorcontrib><creatorcontrib>CHOI, GANG GUK</creatorcontrib><creatorcontrib>YANG, JI WON</creatorcontrib><creatorcontrib>KANG, NAM KYU</creatorcontrib><title>Method for increasing lipid productivity and growth of microalgae by using an overexpression of bHLH transcription factors</title><description>The present invention relates to a recombinant vector, pNs302(bHLH2) which overexpresses a gene (bHLH2) encoding basic helix-loop-helix transcription factor, transgenic microalgae in which the recombinant vector, pNs302(bHLH2), is inserted, and a method for enhancing lipid productivity and a growth rate of microalgae by culturing the microalgae in a nitrogen-depleted medium and/or an osmosis stress medium. The recombinant vector, pNs302(bHLH2), contains a gene (bHLH2) encoding the basic helix-loop-helix transcription factor, tubulin promoter (P_tub) and tubulin terminator (T_tub).
본 발명은 염기성 나선 루프 나선 전사 인자 단백질을 코딩하는 유전자(bHLH2)를 과발현시키는 재조합 벡터 pNs302(bHLH2), 상기 bHLH2(bHLH2)가 삽입되어 있는 형질전환 미세조류 및 상기 미세조류를 질소결핍 배지 및/또는 삼투압 스트레스 배지에서 배양하여 미세조류의 지질 생산성 및 생장률을 증가시키는 방법에 관한 것이다.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2016</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNi8EKgkAQQL10iOofBjoHatI9ohCqS3SXcR11wHaW2dWyrw-jD-j04PHePHpfKbRSQS0KbI0SerYNdOy4AqdS9SbwwGEEtBU0Ks_QgtTwYKOCXYME5Qj9d0ILMpDSyyl5z2KnsMwvOQRF642yC5Ot0QRRv4xmNXaeVj8uovXpeD_kG3JSkHdoyFIozrc0TnZxkqVxmu23_1UfwtxIZA</recordid><startdate>20161212</startdate><enddate>20161212</enddate><creator>KWON, SO HEE</creator><creator>JEONG, BYEONG RYOOL</creator><creator>LEE, BONG SOO</creator><creator>JEON, SEUNG JIB</creator><creator>PARK MIN SUNG</creator><creator>CHOI, GANG GUK</creator><creator>YANG, JI WON</creator><creator>KANG, NAM KYU</creator><scope>EVB</scope></search><sort><creationdate>20161212</creationdate><title>Method for increasing lipid productivity and growth of microalgae by using an overexpression of bHLH transcription factors</title><author>KWON, SO HEE ; JEONG, BYEONG RYOOL ; LEE, BONG SOO ; JEON, SEUNG JIB ; PARK MIN SUNG ; CHOI, GANG GUK ; YANG, JI WON ; KANG, NAM KYU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_KR20160142024A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; kor</language><creationdate>2016</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>KWON, SO HEE</creatorcontrib><creatorcontrib>JEONG, BYEONG RYOOL</creatorcontrib><creatorcontrib>LEE, BONG SOO</creatorcontrib><creatorcontrib>JEON, SEUNG JIB</creatorcontrib><creatorcontrib>PARK MIN SUNG</creatorcontrib><creatorcontrib>CHOI, GANG GUK</creatorcontrib><creatorcontrib>YANG, JI WON</creatorcontrib><creatorcontrib>KANG, NAM KYU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>KWON, SO HEE</au><au>JEONG, BYEONG RYOOL</au><au>LEE, BONG SOO</au><au>JEON, SEUNG JIB</au><au>PARK MIN SUNG</au><au>CHOI, GANG GUK</au><au>YANG, JI WON</au><au>KANG, NAM KYU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for increasing lipid productivity and growth of microalgae by using an overexpression of bHLH transcription factors</title><date>2016-12-12</date><risdate>2016</risdate><abstract>The present invention relates to a recombinant vector, pNs302(bHLH2) which overexpresses a gene (bHLH2) encoding basic helix-loop-helix transcription factor, transgenic microalgae in which the recombinant vector, pNs302(bHLH2), is inserted, and a method for enhancing lipid productivity and a growth rate of microalgae by culturing the microalgae in a nitrogen-depleted medium and/or an osmosis stress medium. The recombinant vector, pNs302(bHLH2), contains a gene (bHLH2) encoding the basic helix-loop-helix transcription factor, tubulin promoter (P_tub) and tubulin terminator (T_tub).
본 발명은 염기성 나선 루프 나선 전사 인자 단백질을 코딩하는 유전자(bHLH2)를 과발현시키는 재조합 벡터 pNs302(bHLH2), 상기 bHLH2(bHLH2)가 삽입되어 있는 형질전환 미세조류 및 상기 미세조류를 질소결핍 배지 및/또는 삼투압 스트레스 배지에서 배양하여 미세조류의 지질 생산성 및 생장률을 증가시키는 방법에 관한 것이다.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for increasing lipid productivity and growth of microalgae by using an overexpression of bHLH transcription factors |
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