Method for increasing lipid productivity and growth of microalgae by using an overexpression of bHLH transcription factors

The present invention relates to a recombinant vector, pNs302(bHLH2) which overexpresses a gene (bHLH2) encoding basic helix-loop-helix transcription factor, transgenic microalgae in which the recombinant vector, pNs302(bHLH2), is inserted, and a method for enhancing lipid productivity and a growth...

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Bibliographische Detailangaben
Hauptverfasser:	KWON, SO HEE, JEONG, BYEONG RYOOL, LEE, BONG SOO, JEON, SEUNG JIB, PARK MIN SUNG, CHOI, GANG GUK, YANG, JI WON, KANG, NAM KYU
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a recombinant vector, pNs302(bHLH2) which overexpresses a gene (bHLH2) encoding basic helix-loop-helix transcription factor, transgenic microalgae in which the recombinant vector, pNs302(bHLH2), is inserted, and a method for enhancing lipid productivity and a growth rate of microalgae by culturing the microalgae in a nitrogen-depleted medium and/or an osmosis stress medium. The recombinant vector, pNs302(bHLH2), contains a gene (bHLH2) encoding the basic helix-loop-helix transcription factor, tubulin promoter (P_tub) and tubulin terminator (T_tub). 본 발명은 염기성 나선 루프 나선 전사 인자 단백질을 코딩하는 유전자(bHLH2)를 과발현시키는 재조합 벡터 pNs302(bHLH2), 상기 bHLH2(bHLH2)가 삽입되어 있는 형질전환 미세조류 및 상기 미세조류를 질소결핍 배지 및/또는 삼투압 스트레스 배지에서 배양하여 미세조류의 지질 생산성 및 생장률을 증가시키는 방법에 관한 것이다.