METHODS FOR RAPID MULTIPLEXED AMPLIFICATION OF TARGET NUCLEIC ACIDS

본 발명은 표적 핵산으로부터 증폭된 핵산 생성물, 예를 들어, 완전한 STR 프로파일을 신속하게 발생시킬 수 있는 신속하고 다중화된 PCR 시스템을 기재한다. 이러한 시스템은, 예를 들어, 미세유체 바이오칩 및 맞춤형(custom built)의 열 순환기를 포함하며, 이 또한 본 발명에 기재되어 있다. 생성된 STR 프로파일은 신호 강도, 유전자좌 간 피크 높이 균형, 이형 피크 높이 비, 불완전한 비-주형 누클레오티드 첨가 및 스터터(stutter)에 대한 법의학적 가이드라인을 만족시킬 수 있다. The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.