VIRUS-DERIVED RNA SILENCING USING TSL MUTATION

The present invention relates to a mechanism which uses TSL (serine/threonine protein kinase, tousled) or a homologous gene thereof from Arabidopsis and other plants to induce the transcription of a virus and to engage specifically in gene silencing. Specifically, TSI mutation can affect extrinsic g...

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Bibliographische Detailangaben
Hauptverfasser:	MOHAMMAD NAZIMUDDIN, KIM, JAE YEAN
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a mechanism which uses TSL (serine/threonine protein kinase, tousled) or a homologous gene thereof from Arabidopsis and other plants to induce the transcription of a virus and to engage specifically in gene silencing. Specifically, TSI mutation can affect extrinsic gene silencing without affecting at all the endogenous production or activation of miRNA or tasiiRNA that plants inherently have, thereby minimizing side effects for RNAi technology. Therefore, the present invention can be usefully employed in inhibiting copying of plant viruses, reducing virus proteins or virus vector-mediated recombination proteins, enhancing the virus resistance of farming goods, and the like. 본 발명은 애기장대() 및 타 식물에서 TSL(serine/threonine protein kinase, TOUSLED ) 또는 그 상동체를 이용하여 외부 유전자 및 바이러스 유도 전사 후 유전자침묵에 특이하게 관여하는 기작에 관한 것으로, 특히, tsl 돌연변이는 식물체가 갖는 고유의 miRNA 또는 tasiRNA 등의 내생성 생산이나 활성에는 전혀 영향을 미치지 않고, 외인성 유전자침묵에 대해서만 영향을 미치는 바, RNAi 기술에 대한 부작용을 최소화 할 수 있어 식물 바이러스의 복제, 바이러스 단백질, 바이러스 벡터 매개 재조합 단백질 또는 농작물의 바이러스 저항성 증대 등에 유용하게 활용될 수 있을 것이다.