RECOMBINANT VECTOR FOR INCREASING BIOMASS AND LIPID PRODUCTIVITY OF MICROALGAE AND USES THEREOF

The present invention relates to a recombination vector characterized by promoter that is induced in the condition without ammonium or with nitrate and that can include protein coding DNA called GAPDH(glyceraldehyde-3-phosphate dehydrogenase) below the promoter, transformed microalgae manufacturing...

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Bibliographische Detailangaben
Hauptverfasser:	KWAK, MIN SU, JEONG, BYEONG RYOOL, JUN, SEUNG JIB, SHIN, SUNG EUN, CHOI, GANG GUK, YANG, JI WON, KANG, NAM KYU
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a recombination vector characterized by promoter that is induced in the condition without ammonium or with nitrate and that can include protein coding DNA called GAPDH(glyceraldehyde-3-phosphate dehydrogenase) below the promoter, transformed microalgae manufacturing method with increased biomass and lipid productivity by performing transformation of microalgae cells by using the recombination vector, composition to increase biomass and lipid productivity of microalgae containing the transformed microalgae manufactured by the method and the recombination vector as active ingredients and a method for producing lipid by using the transformed microalgae and a method for manufacturing biodiesel by using the transformed microalgae. 본 발명은 암모늄이 없는 조건 또는 질산염이 있는 조건에서 유도되는 유도성 프로모터 및 상기 프로모터 하위에 GAPDH(glyceraldehyde-3-phosphate dehydrogenase) 단백질 코딩 유전자를 포함하는 것을 특징으로 하는 재조합 벡터, 상기 재조합 벡터로 미세조류 세포를 형질전환하여 바이오매스와 지질 생산성이 증가된 형질전환 미세조류의 제조 방법, 상기 방법에 의해 제조된 형질전환 미세조류, 상기 재조합 벡터를 유효성분으로 포함하는, 미세조류의 바이오매스와 지질 생산성을 증가시키기 위한 조성물, 상기 형질전환 미세조류를 배양하여 지질을 생산하는 방법 및 상기 형질전환 미세조류를 이용한 바이오디젤의 제조 방법에 관한 것이다