RECOMBINANT VECTOR COMPRISING CYTOCROME P450 REDUCTASE GENES, MICROORGANISM TRANSFORMED THEREOF AND METHOD FOR PRODUCING P450 ENZYME-DERIVED COMPOUNDS USING THE SAME

RECOMBINANT VECTOR COMPRISING CYTOCROME P450 REDUCTASE GENES, MICROORGANISM TRANSFORMED THEREOF AND METHOD FOR PRODUCING P450 ENZYME-DERIVED COMPOUNDS USING THE SAME

The present invention relates to a recombinant vector comprising CPR gene which increases catalytic activity of P450 enzyme, bacteria transformed by the recombinant vector and a recombinant vector comprising P450 gene, and a method for producing a P450 catalysis compound using the same. The recombin...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: PARK, SANG KYU, BACK, KYOUNG WHAN
Format: Patent
Sprache:eng ; kor
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present invention relates to a recombinant vector comprising CPR gene which increases catalytic activity of P450 enzyme, bacteria transformed by the recombinant vector and a recombinant vector comprising P450 gene, and a method for producing a P450 catalysis compound using the same. The recombinant vector according to the present invention comprises a recombinant vector which increases the catalytic activity of P450 enzyme and has a rice-derived NADPH-cytochrome P450 reductase 2 gene consisting of base sequences of sequence number 12. The bacteria according to the present invention comprises: the recombinant vector having the rice-derived NADPH-cytochrome P450 reductase 2 gene consisting of base sequences of sequence number 12; and a recombinant vector comprising tryptamine 5-hydroxylase gene or a recombinant vector comprising cinnamate 4-hydroxylase gene. Serotonin or coumaric acid is stably produced in large amounts using the transformed bacteria.