COMPOSITION FOR DIAGNOSING SEPSIS AND METHOD THEREOF

PURPOSE: A composition and a method for diagnosing sepsis are provided to quickly and accurately identify gram positive-gram negative bacteria including Mycobacterium spp. and fungi including Candida spp. and Aspergillus spp. and to simultaneously identify antibiotic resistance in methicillin resistant staphylococcus aureus (MRSA) and vancomycin resistant enterococci (VRE). CONSTITUTION: A method for providing information for diagnosing sepsis comprises the steps of: isolating DNA from a test sample; amplifying the fragments of 16s rRNA gene, internal transcribed sequence (ITS) gene, gene encoding methicillin resistance in staphylococci (Mec) A gene, and vancomycin resistant protein A and B genes by PCR of the DNA using a primer; and hybridizing a solid support and the PCR product and forming PCR-reverse blot hybrid. The solid support has: an oligomer probe for detecting 16s rRNA gene to distinguish gram positive bacteria and gram negative bacteria; an oligomer probe for detecting ITS gene to distinguish fungi; an oligomer probe for detecting MecA gene to verify antibiotic resistance of MRSA; and an oligomer probe for detecting VanA and VanB genes to verify antibiotic resistance of VRE. A probe composition is selected among a probe for detecting 16s rRNA gene to distinguish gram positive bacteria and gram negative bacteria; a probe for detecting ITS gene to distinguish fungi; a probe for detecting MecA gene to verify antibiotic resistance of MRSA; and a probe for detecting VanA and VanB genes to verify antibiotic resistance of VRE.