METHOD OF PREPARING A THERAPEUTIC HUMAN RECOMBINANT GLUCOCEREBROSIDASE
PURPOSE: A novel suspension cell line which stably produces glucocerebrosidase(GCR enzyme) with a high expression rate is provided to express stabilized GCR enzyme with high efficiency in a serum-free medium, and produce a large amount of GCR enzyme. CONSTITUTION: A recombinant cell line(deposit num...
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| creator | LEE, SUNG HEE KIM, HYUN KI PARK, BEOM SOO KYUNG, YOON JOO NA, KYU HEUM AN, SO RA KIM, SEUNG CHUL |
| description | PURPOSE: A novel suspension cell line which stably produces glucocerebrosidase(GCR enzyme) with a high expression rate is provided to express stabilized GCR enzyme with high efficiency in a serum-free medium, and produce a large amount of GCR enzyme. CONSTITUTION: A recombinant cell line(deposit number KCLRF-BP-00275) is prepared by transforming a vector containing GCR gene with a base sequence number 1 and GIVS gene with a base sequence of sequence number 2. The vector additionally contains DHFR(dihydrofolate reductase) gene. A method for preparing GCR protein comprises a step of suspending and culturing the recombinant cell line in a serum-free medium. |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | METHOD OF PREPARING A THERAPEUTIC HUMAN RECOMBINANT GLUCOCEREBROSIDASE |
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