NOVEL GLUTAMATE DECARBOXYLASE MUTANTS

PURPOSE: A method for preparing GABA(gamma-aminobutyric acid) from novel glutamate decarboxylase(GAD) and high concentration L-glutamate is provided to obtain salt-free gamma-aminobutyric acid. CONSTITUTION: A glutamate decarboxylase mutant is prepared by deleting threonine or adding alanine at carb...

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Bibliographische Detailangaben
Hauptverfasser:	WON, KEE HOON, HO NGOC ANH THU, KANG, TAEK JIN
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMES ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	PURPOSE: A method for preparing GABA(gamma-aminobutyric acid) from novel glutamate decarboxylase(GAD) and high concentration L-glutamate is provided to obtain salt-free gamma-aminobutyric acid. CONSTITUTION: A glutamate decarboxylase mutant is prepared by deleting threonine or adding alanine at carboxy terminal in an amino acid sequence of sequence number 1. The mutant has an affinity tag at an amino terminal. The affinity tag is histidine, maltose binding protein(MBP), thioredoxin(Trx), NusA(N utilization substance A), or glutathione-S-transferase(GST) tag. A method for preparing GABA comprises a step of contacting glutamate or a glutamate salt with the glutamate decarboxylase mutant. The glutamate decarboxylase mutant is fixed on a solid support.