MUTANT BETA-GLUCOSIDASE WITH ADVANCED ACTIVITY AND PROCESS FOR PREPARING BIO-ETHANOL EMPLOYING THE SAME

MUTANT BETA-GLUCOSIDASE WITH ADVANCED ACTIVITY AND PROCESS FOR PREPARING BIO-ETHANOL EMPLOYING THE SAME

PURPOSE: A method for producing bioethanol using variant beta-glucosidase with enhanced activity is provided to produce bioethanol using a transformant. CONSTITUTION: A polynucleotide encodes variant beta-glucosidase with enhanced activity. The variant beta-glucosidase is encoded by a polynucleotide...

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Bibliographische Detailangaben
Hauptverfasser: SOHN, JUNG HOON, SUNG, BONG HYUN, LIM, KWANG MOOK, KIM, HYUN JIN, KIM, CHUL HO, KIM, MI JIN, SEOMOON, JI HUN, KIM, JONG HYUN
Format: Patent
Sprache:eng ; kor
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PURPOSE: A method for producing bioethanol using variant beta-glucosidase with enhanced activity is provided to produce bioethanol using a transformant. CONSTITUTION: A polynucleotide encodes variant beta-glucosidase with enhanced activity. The variant beta-glucosidase is encoded by a polynucleotide comprising: a polynucleotide of a beta-glucosidase gene(sequence number 8) derived from Saccharomycopsis fiburigera, in which 114th base, adenine is substituted to guanine, a polynucleotide in which 1377th, thymine is substituted to cytosine; a polynucleotide in which 1527th base, thymine is substituted to adenine; a nucleotide encoding a polypeptide in which 74th amino acid, threonine is substituted to alanine; a nucleotide encoding a polypeptide in which 275th amino acid, valine is substituted to alanine; or a nucleotide encoding a polypeptide in which 74th and 275th amino acids are substituted to alanine.