AGARASE EXPRESSION VECTOR, TRANSGENIC ORGANISM TRANSFORMED WITH THE SAME VECTOR AND METHOD FOR THE PRODUCTION OF AGARASE USING THE SAME TRANSGENIC ORGANISM

AGARASE EXPRESSION VECTOR, TRANSGENIC ORGANISM TRANSFORMED WITH THE SAME VECTOR AND METHOD FOR THE PRODUCTION OF AGARASE USING THE SAME TRANSGENIC ORGANISM

PURPOSE: A method for preparing agarase using a transformant prepared by an agarase expression vector is provided to simply and effectively obtain agarase. CONSTITUTION: A beta-agarase expression vector is prepared by cloning SCO3487 gene derived from Streptomyces coelicolor A3(2) to a replicable ve...

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Bibliographische Detailangaben
Hauptverfasser: CHI, WON JAE, CHANG, YONG KEUN, UYANGAA TEMUUJIN, HONG, SOON KWANG
Format: Patent
Sprache:eng ; kor
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PURPOSE: A method for preparing agarase using a transformant prepared by an agarase expression vector is provided to simply and effectively obtain agarase. CONSTITUTION: A beta-agarase expression vector is prepared by cloning SCO3487 gene derived from Streptomyces coelicolor A3(2) to a replicable vector to control transcription by ermE promoter. The vector is Puwl201PW cloning vector. A host of a transformant is Streptomyces lividans. The beta-agarase is prepared by culturing the Actinomyces transformant.