METHOD FOR ROBUST EXPANSION OF UMBILICAL CORD BLOOD DERIVED-PLURIPOTENT STEM CELL EXPRESSING ZNF281

METHOD FOR ROBUST EXPANSION OF UMBILICAL CORD BLOOD DERIVED-PLURIPOTENT STEM CELL EXPRESSING ZNF281

PURPOSE: A method for robust expansion of umbilical cord blood derived-pluripotent stem cell expressing znf281 is provided to extend cell growth time by culturing in a container including fibronectin and be used for incurable disease treatment. CONSTITUTION: A stem cell culturing medium includes 1-4...

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Hauptverfasser: ROH, KYOUNG HWAN, KANG, KYUNG SUN
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BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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creator ROH, KYOUNG HWAN
KANG, KYUNG SUN
description PURPOSE: A method for robust expansion of umbilical cord blood derived-pluripotent stem cell expressing znf281 is provided to extend cell growth time by culturing in a container including fibronectin and be used for incurable disease treatment. CONSTITUTION: A stem cell culturing medium includes 1-40nanogram/ml of IGF-I, 1-5 ng/ml of VEGF, EGM-2 which 20-25microgram/ml of heparin is added, or SNU-1 culture medium, fetal bovine serum (FBS), 1-40ng/ml of bFGF, 0.1-5.0microgram/ml of ascorbic acid, 1-40nanogram/ml of EGF, and 0.1-1microgram/ml of hydrocortisone. The fetal bovine serum(FBS) is 20%. The stem cell is an adult stem cell. The stem cell is umbilical cord blood derived-pluripotent stem cell expressing znf281. The stem cell propagating method comprises a step of multiplying stemness by sphere culturing or three dimensional culturing (MEF)mouse embryonic fibroblast cell.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title METHOD FOR ROBUST EXPANSION OF UMBILICAL CORD BLOOD DERIVED-PLURIPOTENT STEM CELL EXPRESSING ZNF281
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