A PURIFICATION METHOD FOR BACTERIOCINS USING ORGANIC SOLVENTS

PURPOSE: A method for extracting and purifying microbes-derived bacteriocin using organic solvent is provided to obtain bacteriocin fraction with 90% or more activity without other purification step. CONSTITUTION: A method for extracting bacteriocin with 10 kDa or less of molecular weight comprises:...

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Hauptverfasser:	LEE, JI EUN, LEE, JONG ARM, JEONG, SEUNG HUN, KIM, HONG JIB, LEE, SUNG TAI, KIM, KI EN, KIM, TAE GUN, JUNG, SANG YOON
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE: A method for extracting and purifying microbes-derived bacteriocin using organic solvent is provided to obtain bacteriocin fraction with 90% or more activity without other purification step. CONSTITUTION: A method for extracting bacteriocin with 10 kDa or less of molecular weight comprises: a step of culturing a bacteriocin-producing strain; a step of centrifuging the culture liquid; a step of adding organic solvent to supernatant and stirring to prepare a mixture; a step of centrifuging the mixture and collecting supernatant containing organic solvent; and a step of concentrating the supernatant to isolate the organic solvent and bacteriocin.