RECOMBINATION EXPRESSION VECTOR TO PRODUCE BETA-AGARASE

RECOMBINATION EXPRESSION VECTOR TO PRODUCE BETA-AGARASE

PURPOSE: A type 2 beta-agarase recombinant expression vector which transforms prokaryote is provided to cheaply produce beta-agarase of high activity. CONSTITUTION: A beta-agarage recombinant expression vector comprises: a Streptomyces griseus-derived trypsin gene(sprT) promoter; and Streptomyces co...

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Bibliographische Detailangaben
Hauptverfasser: CHI, WON JAE, CHANG, YONG KEUN, UYANGAA TEMUUJIN, HONG, SOON KWANG
Format: Patent
Sprache:eng ; kor
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PURPOSE: A type 2 beta-agarase recombinant expression vector which transforms prokaryote is provided to cheaply produce beta-agarase of high activity. CONSTITUTION: A beta-agarage recombinant expression vector comprises: a Streptomyces griseus-derived trypsin gene(sprT) promoter; and Streptomyces coelicolor-derived beta-agarse gene(dagA). The expression vector further contains Streptomyces griseus-derived sgtR1 gene or Streptomyces griseus-derived sgtR2 gene. The recombinant expression vector is pWHM3-TpAsm, pWHM3-TpAsmR1, pWHM3-TpAsmR2, or pWHM3-TpAsmR12. A method for preparing beta-agarase comprises: a step of culturing Actinomyces transformed by the recombinant expression vector; and a step of collecting beta-agarase from the culture.