SSR PRIMER DERIVED FROM AZUKI-BEAN AND USE THEREOF

PURPOSE: An SSR primer isolated from Vigna angularis (Willd.) Ohwi and Ohashi and a method for detecting DNA polymorphism of Vigna angularis (Willd.) Ohwi and Ohashi using the same are provided to effectively detect DNA polymorphism and to determine the product species of Vigna angularis (Willd.) Oh...

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Hauptverfasser: LEE, MYOUNG HEE, PARK, KEUM YONG, SUH, DUCK YONG, AHN, JIN GON, HAN, SANG IK, JUNG, CHAN SIK, HA, TAE JOUNG
Format: Patent
Sprache:eng ; kor
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Zusammenfassung:PURPOSE: An SSR primer isolated from Vigna angularis (Willd.) Ohwi and Ohashi and a method for detecting DNA polymorphism of Vigna angularis (Willd.) Ohwi and Ohashi using the same are provided to effectively detect DNA polymorphism and to determine the product species of Vigna angularis (Willd.) Ohwi and Ohashi. CONSTITUTION: An SSR(simple sequence repeat) primer pair comprises two base sequences selected from sequence numbers 1-12. The SSR primer pair is derived from Vigna angularis (Willd.) Ohwi and Ohashi. A method for detecting DNA polymorphism of Vigna angularis (Willd.) Ohwi and Ohashi comprises: a step of extracting genome DNA from Vigna angularis (Willd.) Ohwi and Ohashi; a step of performing PCR using SSR primer; and a step of performing electrophoresis.