SPECIFIC PRIMERS FOR DISCRIMINATING RICE CULTIVARS, AND USES THEREOF

PURPOSE: A specific primer set for determining rice plant species is provided to shorten PCR time through multiplex PCR using fluorescent primer and to improve accuracy for gene analysis. CONSTITUTION: A primer set for determining rice plant species comprises an oligo nucleotide primer set of sequen...

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Bibliographische Detailangaben
Hauptverfasser:	AN, DEOK JONG, KIM, SE JONG, CHOI, CHUNG DON, WON, JONG GUN
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE: A specific primer set for determining rice plant species is provided to shorten PCR time through multiplex PCR using fluorescent primer and to improve accuracy for gene analysis. CONSTITUTION: A primer set for determining rice plant species comprises an oligo nucleotide primer set of sequence numbers 1 and 2; primer set of sequence numbers 3 and 4; primer set of sequence numbers 5 and 6; or primer set of sequence numbers 7 and 8. The primer set is used for multiplex PCR. A kit for determining rice plant species comprises the oligonucleotide primer set and reagent for PCR. The reagent contains DNA polymerase, dNTPs, and buffer. A method for determining rice plant species comprises: a step of isolating genome DNA from rice plant; a step of amplifying a target sequence by PCR; and a step of detecting the PCR product.