METHOD FOR TRANSFORMATION PROTOCOL OF BASIDIOMYCETE FUNGUS, POLYPORUS BRUMALIS USING PROTOPLAST ISOLATION

PURPOSE: A transformation of P. brumalis through protoplast isolation is provided to improve REMI(restriction enzyme mediated integration) and produce transformant of P. brumalis having enhanced lignin lysis activity. CONSTITUTION: A method for transforming P. brumalis comprises: a step of performin...

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Hauptverfasser:	KIM, MYUNG KIL, RYU, SUN HWA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE: A transformation of P. brumalis through protoplast isolation is provided to improve REMI(restriction enzyme mediated integration) and produce transformant of P. brumalis having enhanced lignin lysis activity. CONSTITUTION: A method for transforming P. brumalis comprises: a step of performing protoplast isolation from P. brumalis mycelium; and a step of adding gene for transforming to the protoplast; and a step of adding cell lysis enzyme liquid to the mycelium of P. brumalis and shaking. The cell wall lysis enzyme liquid contains 0.1-1.0% of USUKizyme based on 0.6M sucrose.