A METHOD FOR PREPARING A COLLAGEN ISOLATED FROM THE SKIN OF THUNNUS OBESUS

PURPOSE: A method for manufacturing collagen isolated from the skin of bigeye tuna is provided to produce high quality of collagen with high solubility and emulsion stability. CONSTITUTION: A method for manufacturing collagen isolated from the skin of bigeye tuna comprises: a step for washing the skin of the bigeye tuna which is stored at -30 °C to -100 °C; a step for treating the skin with 0.05 ~ 10.0 %(w/v) of alkali solution; a step for washing and neutralizing with distilled water; a step for adding 0.05 ~ 10.0 N of acid to the skin, then stirring and extracting; a step for separating extracted liquid and residue using a centrifuge; and a step for drying to obtain acid soluble collagen (AC); a step for adding 0.5 ~ 5.0 N of acid; a step for adding 0.5 ~ 10% of pepsin and stirring and hydrolyzing for 1 to 60 hours; a step for separating extract liquid and residue using a filter; a step for adding NaCl to adjust the concentration to 1-10 % and neutralizing precipitates; and a step for freeze-drying to obtain a pepsin soluble collagen (PC).