A RECOMBINANT VECTOR FOR DELETING SPECIFIC REGIONS OF THE CHROMOSOME AND A METHOD FOR DELETING SPECIFIC CHROMOSOMAL REGIONS OF CHROMOSOME IN THE MICROORGANISM USING THE SAME

A RECOMBINANT VECTOR FOR DELETING SPECIFIC REGIONS OF THE CHROMOSOME AND A METHOD FOR DELETING SPECIFIC CHROMOSOMAL REGIONS OF CHROMOSOME IN THE MICROORGANISM USING THE SAME

A recombinant vector for removing a specific site of chromosome is provided to sequencially and simply remove the specific gene site in a microorganism at once. A recombinant vector for removing a specific site of chromosome comprises a promoter (Para) which is induced by arabinose; a gene which enc...

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Bibliographische Detailangaben
Hauptverfasser: LEE, SANG HEE, YU, BYUNG JO, SUNG, BONG HYUN, PARK, MYUNG KEUN, KIM, SUN CHANG, KANG, KUI HYEON, LEE, JUN HYOUNG, LEE, CHOONG HOON, LEE, JU YOUNG
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A recombinant vector for removing a specific site of chromosome is provided to sequencially and simply remove the specific gene site in a microorganism at once. A recombinant vector for removing a specific site of chromosome comprises a promoter (Para) which is induced by arabinose; a gene which encodes a protein related to lamda-red recombination; and promoter which is induced by a rhamnose. A method for removing the specific site of the chromosome using the recombinant vector comprises: a step of preparing a linear DNA fragment having homologous site A and B which is related to lamda-red recombination, I-SceI restriction enzyme cutting site, and homologous site C which is related to homologous recombination for removing selection marker; a step of introducing linear DNA fragement in transformed microorganism and replacing at specific site of microorganism chromosome; and a step of expressing I-SceI restriction enzyme and removing selection marker.