MULTIPLEX REAL TIME PCR METHOD FOR DISCRIMINATION OF RICE CULTIVAR

A real time multiplex polymerase chain reaction is provided to minimize faults by operating the reaction automatically and to analyze and collect data by analyzing species of rice plants with a plurality of markers at the same time. A marker composition distinguishing species of rice plants includes...

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Bibliographische Detailangaben
Hauptverfasser:	JUNG, JIN KEE, KIM, IK CHUL, CHOI, JEONG A, NAM, YONG SUK
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A real time multiplex polymerase chain reaction is provided to minimize faults by operating the reaction automatically and to analyze and collect data by analyzing species of rice plants with a plurality of markers at the same time. A marker composition distinguishing species of rice plants includes a set of makers including two primers and one probe two or more. A method for distinguishing species of rice plants using marker sets for a plurality of species of the rice plants comprises the following steps of: performing a PCR in real time with DNA extracted from the rice plants using the marker composition; confirming PCR amplification by sets of makers after detecting fluorescent material peak obtained from a result of the PCR; and distinguishing the species of the rice plants according to amplification of the PCR.