METHOD OF DETECTING CEREUS STRAIN PRODUCING EMETIC TOXIN AND PRIMER SET FOR THE METHOD

It is intended to provide a method whereby a cereus strain producing an emetic toxin which has an emetic toxin synthase gene but is negative in an animal cell vacuolation test (i.e., being so-called false positive) can be discriminated and thus a cereus strain being positive in the animal cell vacuo...

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Bibliographische Detailangaben
Hauptverfasser:	MIYAMOTO TAKAHISA, KAMIKADO HIDEAKI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	It is intended to provide a method whereby a cereus strain producing an emetic toxin which has an emetic toxin synthase gene but is negative in an animal cell vacuolation test (i.e., being so-called false positive) can be discriminated and thus a cereus strain being positive in the animal cell vacuolation test alone can be surely detected. More specifically, an emetic toxin-producing cereus strain can be distinguished from an emetic toxin non-producing cereus strain based on the production curves of nucleic acids which are amplified and produced by conducting RT-PCR with the use of nucleic acids respectively having the base sequence represented by SEQ ID NO:1 and the base sequence represented by SEQ ID NO:2 as primers.