CELL-BASED ANTIBODY DETECTION METHOD

A method for detecting and quantitating an antibody is provided to make it possible to detect rapidly, conveniently and accurately an antibody against an antigen which is hard to be obtained through purification by using a host cell transfected by a recombinant vector which is constructed to express the antigen on the surface of an animal cell or in the cell. A method for detecting and quantitating an antibody comprises the steps of: (a) transfecting a host cell such as an animal cell, a plant cell or an insect cell using a nucleotide coding an antigen protein and a nucleotide coding a reporter protein such as EGFP(Enhanced green fluorescence protein), ERFP(Enhanced red fluorescence protein), EBFP(Enhanced blue fluorescence protein), EYFP(Enhanced yellow fluorescence protein), ECFP(Enhanced cyan fluorescence protein), GFP(Green fluorescence protein), RFP(Red fluorescence protein), BFP(Blue fluorescence protein), YFP(Yellow fluorescence protein) and CFP(cyan fluorescence protein) to detect an antibody specifically coupled to the antigen such as HA(haemagglutinin), Tie2(receptor protein tyrosine kinase), gankyrin, KIF14(kinesin family member 14), TM7SF3(transmembrane 7 superfamily member 3) and KIAA0152(KIAA 0152 gene product); (b) culturing the transected host cell; (c) contacting the cultured host cell with a biological sample such as serum, blood, urine, saliva, pancreatin, cerebrospinal fluid and semen; and (c) measuring antigen-antibody reaction.