STATIONARY PHASE AND COLUMN USING CUCURBITURIL BONDED SILICA GEL, AND SEPARATION METHOD OF TAXOL USING THE COLUMN

A stationary phase and column by using cucurbituril bonded silica gel are provided to separate high purity of taxane from low purity of taxane extract and reduce the costs for purification of taxane, so that high purity of taxane and paclitaxel isolated therefrom are useful for treating cancer. A stationary phase contains cucurbituril represented by the formula(1) or formula(2), in which R1 and R2 are each independently hydrogen, hydroxyl group, C1-C30 alkyloxy group, C1-C30 alkynyloxy group, C2-C30 carbonylalkyloxy group, C1-C30 thioalkyloxy group, C1-C30 alkylthioloxy group, C1-C30 hydroxyalkyloxy group, C1-C30 alkylsiloxy group, C1-C30 aminoalkyloxy group, C1-C30 aminoalkylthioalkyloxy group, C5-C30 cycloalkyloxy group, C2-C30 heterocycloalkyloxy group, C6-C30 aryloxy group, C6-C20 arylalkyloxy group, C4-C30 heteroaryloxy group, C4-C30 heteroarylalkyloxy group, C1-C30 alkylthio group, C1-C30 alkenylthio group, C1-C30alkynylthio group, C2-C30 carbonylalkylthio group, C1-C30 thioalkylthio group, C1-C30 hydroxyalkylthio group, C1-C30 alkylsilylthio group, C1-C30 aminoalkylthio group, C1-C30 aminoalkylthiolalkylthio group, C5-C30 cycloalkylthio group, C2-C30 heterocycloalkylthio group, C6-C30 arylthio group, C6-C20 arylalkylthio group, C4-C30heteroarylthio group, C4-C30 heteroarylalkylthio group, C1-C30 alkylamine group, C1-C30 alkenylamine group, C1-C30 alkynylamine group, C2-C30 carbonylalkylamine group, C1-C30 thioalkylamine group, C1-C30 hydroxyalkylamine group, C1-C30 alkylsilylamine group, C1-C30 trialkyloxysilyl alkyl-carbamate group, C1-C30 trialkyloxysilyl alkyl-thiocarbamate group, C1-C30 trialkyloxysilyl alkyloxy group, C1-C30 trialkyloxysilyl alkyl-carbonyloxy group, C1-C30 trialkyloxysilyl heteroalkyl-alkyloxy group, C1-C30 aminoalkylamine group, C1-C30 aminoalkylthioalkylamine group, C5-C30 cycloalkylamine group, C2-C30 heterocycloalkylamine group, C6-C30 arylamine group, C6-C20 arylalkylamine group, C4-C30 heteroarylamine, C4-C30 heteroarylamine group, C1-C30 alkylazide group, C1-C30 arylazide group, C1-C30 alkylcarboxylazide group or C1-C30 arylcarboxylazide group; X is O, S or NH; and n is an integer from 4 to 20, which is covalently bonded to silica gel represented by the formula(3), in which R2 is C2-C10 alkylthiol group, C2-C10 alkylamine group, C2-C10 epoxyalkyloxyalkyl group, C2-C10 isocyanatoalkyl group, C2-C10 halogenated alkyl group or hydroxyl group.