NOVEL N-ACETYLGLUCOSAMINE-2-EPIMERASE AND METHOD FOR PRODUCING CMP-NEURAMINIC ACID USING THE SAME

A novel N-acetylglucosamine-2-epimerase is provided to produce a large quantity of CMP(cytidine monophosphate)-N-acetylneuraminic acid economically through one reaction by using inexpensive substrates of cytidine monophosphate and N-acetyl-D-glucosamine. A novel N-acetylglucosamine-2-epimerase has t...

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Hauptverfasser:	SOHNG, JAE KYUNG, GIL, TAE GEON, SEO, WON MIN, WOO, JIN SUK, KIM, BYUNG GEE, SHIM, SANG HEE, YANG, JI YOUNG, KIM, DEA HEE, JANG, KYOUNG SOON, HUR, IN KANG, KANG, SUN YOUP
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	A novel N-acetylglucosamine-2-epimerase is provided to produce a large quantity of CMP(cytidine monophosphate)-N-acetylneuraminic acid economically through one reaction by using inexpensive substrates of cytidine monophosphate and N-acetyl-D-glucosamine. A novel N-acetylglucosamine-2-epimerase has the amino acid sequence of SEQ ID NO:2. A gene encoding the N-acetylglucosamine-2-epimerase has the nucleotide sequence of SEQ ID NO:1. The N-acetyl-D-mannosamine is produced by reacting the reaction mixture containing N-acetyl-D-glucosamine in the presence of N-acetylglucosamine-2-epimerase. The neuraminic acid is produced by reacting the reaction mixture containing N-acetyl-D-mannosamine in the presence of N-acetylneuraminic acid aldolase. The CMP-N-acetylneuraminic acid is produced by reacting a reaction mixture containing cytidine 5' monophosphate(CMP), nucleotide tri-phosphate(NTP) or ATP, acetyl phosphate(acetyl Pi), N-acetyl-D-glucosamine and pyruvic acid in the presence of N-acetylglucosamine-2-epimerase(GlcNAc 2-epimerase), N-acetylneuraminic acid aldolase(NeuNAc aldolase), acetate kinase and CMP-N- acetylneuraminic acid synthetase.