PRODUCTION OF TRANSGENIC CLONED EMBRYO BY SOMATIC CELL NUCLEUS TRANSFECTED WITH HGM-CSF GENE AND PREPARATION METHOD OF THE SAME

PRODUCTION OF TRANSGENIC CLONED EMBRYO BY SOMATIC CELL NUCLEUS TRANSFECTED WITH HGM-CSF GENE AND PREPARATION METHOD OF THE SAME

A production method of a transgenic cloned embryo having a goat somatic cell nucleus transfected with hGM-CSF(human granulocyte macrophage-colony stimulating factor) gene is provided to produce a cloned goat expressing the hGM-CSF from the mammary gland, thereby improving the production yield of hGM...

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Bibliographische Detailangaben
Hauptverfasser: PARK, SUNG HO, LEE, SUNG HOON, HEO, KI NAM, KIM, TAE SHUK, PARK, JUN KYU, PARK, KWANG WOOK, PARK, HEE SUNG, PARK, BUN YOUNG, JUNG, SOO YOUNG, HONG, SEUNG PYO, SEOL, JAE KOO, CHOI, KI MYUNG
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A production method of a transgenic cloned embryo having a goat somatic cell nucleus transfected with hGM-CSF(human granulocyte macrophage-colony stimulating factor) gene is provided to produce a cloned goat expressing the hGM-CSF from the mammary gland, thereby improving the production yield of hGM-CSF. A production method of a transgenic cloned goat embryo comprises the steps of: (1) transforming a somatic cell of goat with a recombinant expression vector containing an hGM-CSF gene to prepare a nucleus donor cell; (2) collecting an ovum from the goat to prepare a nucleus recipient ovum; and (3) denucleating the recipient ovum, transplanting the nucleus donor cell into the recipient ovum and fusing them to prepare the transgenic cloned embryo.