DNA CHIP FOR DETERMINING OF PHARMACEUTICAL DRUG WITH HEPATOTOXICITY

A DNA chip for determining a pharmaceutical drug with hepatotoxicity is provided to improve rapidness and accuracy of determination by specifically detecting changes of gene expression patterns upon administration of a pharmaceutical drug with hepatotoxicity. A DNA chip for determining a pharmaceuti...

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Hauptverfasser:	KIM, SEUNG JUN, KANG, KYUNG SUN, HWANG, SEUNG YONG, PARK, JOON SUK, KIM, YONG HYUN, LEE, CHANG HYEON, YEOM, HYE JUNG, HWANG, JAE WOONG, YEO, CHAN DONG, UM, CHAN HWI, LEE, GYOUNG JAE, YOUN, JONG PIL, KANG, JONG SOO, LEE, YONG SOON, JEON, KI SEON, JEON, KUM JIN, SONG, BOK SOO, OH, MOON JU, JUNG, JIN WOOK, YOON, HYUN KYU, KIM, JI HOON, KIM, YANG SEOK, LEE, WAN SEON, KIM, JUN SUB
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A DNA chip for determining a pharmaceutical drug with hepatotoxicity is provided to improve rapidness and accuracy of determination by specifically detecting changes of gene expression patterns upon administration of a pharmaceutical drug with hepatotoxicity. A DNA chip for determining a pharmaceutical drug with hepatotoxicity comprises (i) a gene probe capable of specifically binding to a gene of which expression pattern is changed when a pharmaceutical drug with hepatotoxicity is administered, (ii) a linker containing oligo(dT)15, -(CH2)6- and amine group, sequentially and being linked to the 5'-terminal of the gene probe through the thymine site, and (iii) a solid substrate having an aldehyde group on the surface and being linked to the linker through Schiff's base binding between an amine group of linker and aldehyde group of the substrate, wherein the gene probe has the nucleotide sequence selected from SEQ ID NO:1 to SEQ ID NO:54.