5 Primer Combination for Simultaneous Detection of Five Cucurbit-infecting Viruses

PURPOSE: A primer combination for simultaneously detecting five cucurbit-infecting viruses is provided, thereby increasing the detection limitation and sensitivity about 1,000 times higher than using antiserum, and simultaneously detecting all kinds of cucurbit-infecting viruses. CONSTITUTION: The primer combination for simultaneously detecting five cucurbit-infecting viruses including cucumber mosaic virus(CMV), cucumber green mottle mosaic virus(CGMMV), kyuri green mottle mosaic virus(CGMMV), watermelon mosaic virus 2(WMV2) and zucchini yellow mosaic virus(ZYMV) comprises a combination primers consisting of the nucleotide sequences set forth in SEQ ID NOs: 5 and 11, the nucleotide sequences set forth in SEQ ID NOs: 15 and 22, the nucleotide sequences set forth in SEQ ID NOs: 27 and 29, the nucleotide sequences set forth in SEQ ID NOs: 34 and 40, and the nucleotide sequences set forth in SEQ ID NOs: 49 and 51, complementary nucleotide sequences with the nucleotide sequence of the combination primers, and mutated nucleotide sequences thereof, wherein the mutation is selected from deletion of base, addition of base, substitution of base or the combination thereof. 본 발명은 오이모자이크바이러스, 오이녹반모자이크바이러스, 큐리녹반모자이크바이러스, 수박모자이크바이러스2 및 쥬키니황화모자이크바이러스를 동시진단하기 위한 프라이머 조합을 개시한다. 본 발명에 의하면 항혈청을 이용한 진단방법보다 1,000배 이상 검출한계가 높으며, 지금까지 알려진 박과작물 5종 바이러스의 모든 계통에 대한 동시검출이 가능하다.