Alcohol resistant medium for Yeast Saccharomyces cerevisiae and high concentration cultivation of biomass

PURPOSE: An alcohol resistant medium composition for yeasts and a method for culturing yeast cells therein medium in higher yield are provided. The alcohol fermentation is mainly occurred under anaerobic condition and cultivation of yeast cells is mainly occurred under aerobic condition. The raw materials are allowed as food additives, so that post-treatment of products is not required. CONSTITUTION: The alcohol resistant medium composition for yeasts contains defatted milk as a nitrogen source, high concentration of sugar, which inhibits infection of various bacteria and induces increase of alcohol yield, and yeast extract, wherein the yeast is Saccharomyces cerevisiae(KCTC 7107) or (KCTC 7928). The alcohol fermentation or the cultivation of yeast cells is independently carried out by anaerobically or aerobically culturing Saccharomyces cerevisiae(KCTC 7107) or (KCTC 7928) in the alcohol resistant medium composition in a batch reactor at 25 to 35 deg. C for 1 to 3 days. 본 발명은 알코올 발효 효모균주들을 이용하여 고안한 알코올 저항성 배지조성에 관한 것으로, 그 조성은 식품위생등급의 소재만을 사용한 완충력을 지닌 복합배지조성으로서 탈지분유를 질소원으로 하고, 설탕을 탄소원으로 하였으며, 생육인자로서는 효모추출물을 사용한 것이다. 이는 혐기적 조건하에서 효모의 생존율을 증대시킨 알코올 발효를 주로 수행케 하고, 호기적 조건하에서 우유단백질을 미생물에 의해 전분해물을 함유한 균체의 고효율 배양에 있어서의 목적달성을 가능케 하였다. [색인어] 효모, 설탕, 알코올 발효, 탈지분유, 전분해, 알코올 저항성, 균체, 고효율,, skim milk.