Novel Thrombolytic Protein and a Process for Preparing the Same
PURPOSE: A thrombolytic protein and its preparation process are provided, therefore the prepared thrombolytic protein can be used as anti-thrombolytic agent without side-effects. CONSTITUTION: The thrombolytic protein, tempase, has the amino acid sequence of SEQ ID NO: 1; the molecular weight of 38...
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| creator | JUNG, GWANG HOE KWON, SEUNG TAEK YOON, SEON JU HWANG, JAE GWAN PYUN, YU RYANG YEO, IK HYEON SIM, GWAN SEOP |
| description | PURPOSE: A thrombolytic protein and its preparation process are provided, therefore the prepared thrombolytic protein can be used as anti-thrombolytic agent without side-effects. CONSTITUTION: The thrombolytic protein, tempase, has the amino acid sequence of SEQ ID NO: 1; the molecular weight of 38 kDa by SDS-PAGE or 39 kDa by HPLC gel filtration analysis; and the isoelectric point of pH 4.8; and maximum fibrinolytic activity at pH 7.0 to 9.5 and is Ca2+-dependent metal protease. It is produced from Enterococcus faecalis BRCA-5(KFCC-11183). The process for preparing the thrombolytic protein comprises the steps of: cultivating Enterococcus faecalis BRCA-5(KFCC-11183) at 25 to 40 deg.C and pH 5.0 to 9.0; and subjecting the cultivated medium to ion exchange chromatography and gel chromatography.
본 발명은 엔테로코커스 훼컬리스 BRCA-5(BRCA-5, KFCC-11183)로부터 분리되며, 혈전형성 단백질에 특이적으로 작용하는 템파제와 전기 단백질을 효율적으로 생산하는 방법을 제공한다. 본 발명에 의하면, 엔테로코커스 훼컬리스 BRCA-5를 25℃ 내지 40℃의 온도범위에서, pH 5.0 내지 9.0의 조건으로 배양하고, 이로부터 템파제를 수득하는 단계를 포함하는 방법에 의하여 템파제를 제조할 수 있으며, 전기 제조된 템파제는 강력한 혈전용해 활성을 가질뿐만 아니라, 독성 또는 위장장애 등의 부작용이 없으므로, 항혈전제의 유효성분으로 안전하게 사용될 수 있을 뿐 아니라, 전기 엔테로코커스 훼컬리스 BRCA-5 균주 배양액 또는 템파제를 건강식품에 첨가하여 사용할 수 있다. |
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본 발명은 엔테로코커스 훼컬리스 BRCA-5(BRCA-5, KFCC-11183)로부터 분리되며, 혈전형성 단백질에 특이적으로 작용하는 템파제와 전기 단백질을 효율적으로 생산하는 방법을 제공한다. 본 발명에 의하면, 엔테로코커스 훼컬리스 BRCA-5를 25℃ 내지 40℃의 온도범위에서, pH 5.0 내지 9.0의 조건으로 배양하고, 이로부터 템파제를 수득하는 단계를 포함하는 방법에 의하여 템파제를 제조할 수 있으며, 전기 제조된 템파제는 강력한 혈전용해 활성을 가질뿐만 아니라, 독성 또는 위장장애 등의 부작용이 없으므로, 항혈전제의 유효성분으로 안전하게 사용될 수 있을 뿐 아니라, 전기 엔테로코커스 훼컬리스 BRCA-5 균주 배양액 또는 템파제를 건강식품에 첨가하여 사용할 수 있다.</description><edition>7</edition><language>eng ; kor</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2002</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20020201&DB=EPODOC&CC=KR&NR=20020009017A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20020201&DB=EPODOC&CC=KR&NR=20020009017A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>JUNG, GWANG HOE</creatorcontrib><creatorcontrib>KWON, SEUNG TAEK</creatorcontrib><creatorcontrib>YOON, SEON JU</creatorcontrib><creatorcontrib>HWANG, JAE GWAN</creatorcontrib><creatorcontrib>PYUN, YU RYANG</creatorcontrib><creatorcontrib>YEO, IK HYEON</creatorcontrib><creatorcontrib>SIM, GWAN SEOP</creatorcontrib><title>Novel Thrombolytic Protein and a Process for Preparing the Same</title><description>PURPOSE: A thrombolytic protein and its preparation process are provided, therefore the prepared thrombolytic protein can be used as anti-thrombolytic agent without side-effects. CONSTITUTION: The thrombolytic protein, tempase, has the amino acid sequence of SEQ ID NO: 1; the molecular weight of 38 kDa by SDS-PAGE or 39 kDa by HPLC gel filtration analysis; and the isoelectric point of pH 4.8; and maximum fibrinolytic activity at pH 7.0 to 9.5 and is Ca2+-dependent metal protease. It is produced from Enterococcus faecalis BRCA-5(KFCC-11183). The process for preparing the thrombolytic protein comprises the steps of: cultivating Enterococcus faecalis BRCA-5(KFCC-11183) at 25 to 40 deg.C and pH 5.0 to 9.0; and subjecting the cultivated medium to ion exchange chromatography and gel chromatography.
본 발명은 엔테로코커스 훼컬리스 BRCA-5(BRCA-5, KFCC-11183)로부터 분리되며, 혈전형성 단백질에 특이적으로 작용하는 템파제와 전기 단백질을 효율적으로 생산하는 방법을 제공한다. 본 발명에 의하면, 엔테로코커스 훼컬리스 BRCA-5를 25℃ 내지 40℃의 온도범위에서, pH 5.0 내지 9.0의 조건으로 배양하고, 이로부터 템파제를 수득하는 단계를 포함하는 방법에 의하여 템파제를 제조할 수 있으며, 전기 제조된 템파제는 강력한 혈전용해 활성을 가질뿐만 아니라, 독성 또는 위장장애 등의 부작용이 없으므로, 항혈전제의 유효성분으로 안전하게 사용될 수 있을 뿐 아니라, 전기 엔테로코커스 훼컬리스 BRCA-5 균주 배양액 또는 템파제를 건강식품에 첨가하여 사용할 수 있다.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2002</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLD3yy9LzVEIySjKz03Kz6ksyUxWCCjKL0nNzFNIzEtRSATxklOLixXS8ouA7NSCxKLMvHSFkoxUheDE3FQeBta0xJziVF4ozc2g7OYa4uyhm1qQH59aXJCYnJqXWhLvHWRkYABEBpYGhuaOxsSpAgATZjEa</recordid><startdate>20020201</startdate><enddate>20020201</enddate><creator>JUNG, GWANG HOE</creator><creator>KWON, SEUNG TAEK</creator><creator>YOON, SEON JU</creator><creator>HWANG, JAE GWAN</creator><creator>PYUN, YU RYANG</creator><creator>YEO, IK HYEON</creator><creator>SIM, GWAN SEOP</creator><scope>EVB</scope></search><sort><creationdate>20020201</creationdate><title>Novel Thrombolytic Protein and a Process for Preparing the Same</title><author>JUNG, GWANG HOE ; KWON, SEUNG TAEK ; YOON, SEON JU ; HWANG, JAE GWAN ; PYUN, YU RYANG ; YEO, IK HYEON ; SIM, GWAN SEOP</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_KR20020009017A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; kor</language><creationdate>2002</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>JUNG, GWANG HOE</creatorcontrib><creatorcontrib>KWON, SEUNG TAEK</creatorcontrib><creatorcontrib>YOON, SEON JU</creatorcontrib><creatorcontrib>HWANG, JAE GWAN</creatorcontrib><creatorcontrib>PYUN, YU RYANG</creatorcontrib><creatorcontrib>YEO, IK HYEON</creatorcontrib><creatorcontrib>SIM, GWAN SEOP</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>JUNG, GWANG HOE</au><au>KWON, SEUNG TAEK</au><au>YOON, SEON JU</au><au>HWANG, JAE GWAN</au><au>PYUN, YU RYANG</au><au>YEO, IK HYEON</au><au>SIM, GWAN SEOP</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Novel Thrombolytic Protein and a Process for Preparing the Same</title><date>2002-02-01</date><risdate>2002</risdate><abstract>PURPOSE: A thrombolytic protein and its preparation process are provided, therefore the prepared thrombolytic protein can be used as anti-thrombolytic agent without side-effects. CONSTITUTION: The thrombolytic protein, tempase, has the amino acid sequence of SEQ ID NO: 1; the molecular weight of 38 kDa by SDS-PAGE or 39 kDa by HPLC gel filtration analysis; and the isoelectric point of pH 4.8; and maximum fibrinolytic activity at pH 7.0 to 9.5 and is Ca2+-dependent metal protease. It is produced from Enterococcus faecalis BRCA-5(KFCC-11183). The process for preparing the thrombolytic protein comprises the steps of: cultivating Enterococcus faecalis BRCA-5(KFCC-11183) at 25 to 40 deg.C and pH 5.0 to 9.0; and subjecting the cultivated medium to ion exchange chromatography and gel chromatography.
본 발명은 엔테로코커스 훼컬리스 BRCA-5(BRCA-5, KFCC-11183)로부터 분리되며, 혈전형성 단백질에 특이적으로 작용하는 템파제와 전기 단백질을 효율적으로 생산하는 방법을 제공한다. 본 발명에 의하면, 엔테로코커스 훼컬리스 BRCA-5를 25℃ 내지 40℃의 온도범위에서, pH 5.0 내지 9.0의 조건으로 배양하고, 이로부터 템파제를 수득하는 단계를 포함하는 방법에 의하여 템파제를 제조할 수 있으며, 전기 제조된 템파제는 강력한 혈전용해 활성을 가질뿐만 아니라, 독성 또는 위장장애 등의 부작용이 없으므로, 항혈전제의 유효성분으로 안전하게 사용될 수 있을 뿐 아니라, 전기 엔테로코커스 훼컬리스 BRCA-5 균주 배양액 또는 템파제를 건강식품에 첨가하여 사용할 수 있다.</abstract><edition>7</edition><oa>free_for_read</oa></addata></record> |
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| title | Novel Thrombolytic Protein and a Process for Preparing the Same |
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