Novel Thrombolytic Protein and a Process for Preparing the Same

Novel Thrombolytic Protein and a Process for Preparing the Same

PURPOSE: A thrombolytic protein and its preparation process are provided, therefore the prepared thrombolytic protein can be used as anti-thrombolytic agent without side-effects. CONSTITUTION: The thrombolytic protein, tempase, has the amino acid sequence of SEQ ID NO: 1; the molecular weight of 38...

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Hauptverfasser: JUNG, GWANG HOE, KWON, SEUNG TAEK, YOON, SEON JU, HWANG, JAE GWAN, PYUN, YU RYANG, YEO, IK HYEON, SIM, GWAN SEOP
Format: Patent
Sprache:eng ; kor
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PURPOSE: A thrombolytic protein and its preparation process are provided, therefore the prepared thrombolytic protein can be used as anti-thrombolytic agent without side-effects. CONSTITUTION: The thrombolytic protein, tempase, has the amino acid sequence of SEQ ID NO: 1; the molecular weight of 38 kDa by SDS-PAGE or 39 kDa by HPLC gel filtration analysis; and the isoelectric point of pH 4.8; and maximum fibrinolytic activity at pH 7.0 to 9.5 and is Ca2+-dependent metal protease. It is produced from Enterococcus faecalis BRCA-5(KFCC-11183). The process for preparing the thrombolytic protein comprises the steps of: cultivating Enterococcus faecalis BRCA-5(KFCC-11183) at 25 to 40 deg.C and pH 5.0 to 9.0; and subjecting the cultivated medium to ion exchange chromatography and gel chromatography. 본 발명은 엔테로코커스 훼컬리스 BRCA-5(BRCA-5, KFCC-11183)로부터 분리되며, 혈전형성 단백질에 특이적으로 작용하는 템파제와 전기 단백질을 효율적으로 생산하는 방법을 제공한다. 본 발명에 의하면, 엔테로코커스 훼컬리스 BRCA-5를 25℃ 내지 40℃의 온도범위에서, pH 5.0 내지 9.0의 조건으로 배양하고, 이로부터 템파제를 수득하는 단계를 포함하는 방법에 의하여 템파제를 제조할 수 있으며, 전기 제조된 템파제는 강력한 혈전용해 활성을 가질뿐만 아니라, 독성 또는 위장장애 등의 부작용이 없으므로, 항혈전제의 유효성분으로 안전하게 사용될 수 있을 뿐 아니라, 전기 엔테로코커스 훼컬리스 BRCA-5 균주 배양액 또는 템파제를 건강식품에 첨가하여 사용할 수 있다.