SINGLE CHAIN-HUMAN CHORIONIC GONADOTROPIN (HCG) AND PREPARATION METHOD THEREOF

PURPOSE: A human chorionic gonadotropin (hcg) consisting of single chain by genetic recombination is provided, which has same physiological activity as natural type human chorionic gonadotropin (hcg), and is thus used for animal medicines and clinical medicines. Also, nucleic acid molecule coding the hormone and its preparation method are provided. CONSTITUTION: A single chain-human chorionic gonadotropin (hcg) is composed of α-unit and β-unit and is identical with or similar to 1-257 site or 21-257 site of sequence list 6. A single chain-human chorionic gonadotropin (hcg) is prepared by the following steps of: (a) amplifying nucleic acids coding α and β units of human chorionic gonadotropin (hgc) by PCR; (b) cloning amplified nucleic acids coding α and β units; (c) coupling the resultant nucleic acids; (d) constructing an expression vector including the coupled nucleic acid; (e) transforming an eukaryotic cell using the expression vector; (f) expressing human chorionic gonadotropin in the eukaryotic cell; and (g) separating the prepared human chorionic gonadotropin.