SEPARATING METHOD OF GLYCOSYLATED PROTEINS AND NON-GLYCOSYLATED PROTEINS

PURPOSE: A separating method of glycosylated proteins and non-glycosylated proteins is provided, bywhich human albumins that are revealed in yeast cell not comprising glycoside binding can be manufactured. CONSTITUTION: A separating process of glycosylated proteins and non-glycosylated proteins comp...

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Hauptverfasser:	ANNETTE FEUSNER, HARALD STAUS, JOERG WEISSE, JUERGEN ROEMISCH
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE: A separating method of glycosylated proteins and non-glycosylated proteins is provided, bywhich human albumins that are revealed in yeast cell not comprising glycoside binding can be manufactured. CONSTITUTION: A separating process of glycosylated proteins and non-glycosylated proteins comprises steps of: treating triazine dye fixed on matrix at constant temperature with a mixture of glycosylated proteins and non-glycosylated proteins; washing the matrix to eliminate non-bound proteins; and dissolving proteins out by selected conditions to dissolve each bound proteins out like as increasing ionic strength or pH gradually or continuously, or making glycosylated proteins and non-glycosylated proteins to pass through the non-bound matrix; so finding the partial or complete separating process of glycosylated proteins and non-glycosylated proteins that are collected separately from gained dissolved fraction.