VECTOR CONTAINING SUPEROXIDE DISMUTASE AND ASCORBATE PEROXIDASE GENE AND TRANSFORMED PLANT WITH IT

PURPOSE: The aim is to provide transformed plant having resistance of environmental stress by using vector containing superoxide dismutase(SOD) and as corbate peroxidase(APX). CONSTITUTION: For practicing example of preparing vector, cDNA of MnSOD and APX is prepared and inserted in between 35S prom...

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Hauptverfasser:	LEE, HAENG SOON, KWON, SEOK YOON, LANDYALLEN, KWAG, SANG SU
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PURPOSE: The aim is to provide transformed plant having resistance of environmental stress by using vector containing superoxide dismutase(SOD) and as corbate peroxidase(APX). CONSTITUTION: For practicing example of preparing vector, cDNA of MnSOD and APX is prepared and inserted in between 35S promoter and 35S terminator of cauliflower mosaic virus(pCGN1578). It is cut with HindIII to produce about 1.7kb-1.5kb of DNA fragments. pGPTV-Bar is used as transforming vector for double transformation. GUS gene of pGPTV-Bar is removed by using EcoR1 and SmaI and the fragment containing SOD and APX is inserted into the vector. Plasmid DNA of the prepared vector is introduced to Agrobaterium tumefaciens(LBA4404).