CRISPR/dCas9 CRISPR/dCas9-mediated Surface-enhanced Raman Scattering Assay for Detecting Pathogen

The present invention relates to a surface-enhanced Raman scattering-based pathogen detection method using CRISPR/dCas9. The pathogen detection method according to the present invention can quickly and sensitively detect a target pathogen gene without a separate gene separation and amplification ste...

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Bibliographische Detailangaben
Hauptverfasser:	SEO HWI WON, KANG BYUNGHOON, MOON JEONG, KIM HONGKI, LIM EUN KYUNG, RYU CHOONGMIN, KANG TAEJOON, KANG HYUNJU, JUNG JUYEON, LEE SOOHYUN
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a surface-enhanced Raman scattering-based pathogen detection method using CRISPR/dCas9. The pathogen detection method according to the present invention can quickly and sensitively detect a target pathogen gene without a separate gene separation and amplification step, and can provide rapid diagnostic information through excellent specificity and rapidity. 본 발명은 CRISPR/dCas9을 이용한 표면증강라만산란 기반 병원균 검출법에 관한 것이다. 본 발명에 따른 병원균 검출 방법은 별도의 유전자 분리 및 증폭 단계 없이 신속하고 민감하게 표적 병원균의 유전자를 검출할 수 있고, 우수한 특이성 및 신속성을 통하여 빠른 진단 정보를 제공할 수 있다.