a Method for isolating and purifying chlorophyll a from microalgae
The present invention relates to a method for separating and purifying high purity chlorophyll a from microalgae. More particularly, the present invention relates to a method capable of purifying chlorophyll a with a purity of 90% or more in a short time, wherein chlorophyll a is extracted from micr...
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| creator | CHOI JIN YOUNG CHOI, TAE O |
| description | The present invention relates to a method for separating and purifying high purity chlorophyll a from microalgae. More particularly, the present invention relates to a method capable of purifying chlorophyll a with a purity of 90% or more in a short time, wherein chlorophyll a is extracted from microalgae using ethanol and hexane, and then first purified using a column packed with Sephadex resin; and subjected to secondary purification using a column packed with C18 resin sequentially. Chlorophyll a purified by the separation and purification method of the present invention can be usefully used as a material for antioxidant cosmetics or functional health foods for antioxidants.
본 발명은 미세조류로부터 고순도 클로로필 a를 분리 및 정제하는 방법에 관한 것으로, 보다 상세하게는 에탄올 및 헥산을 이용하여 미세조류로부터 클로로필 a를 추출한 후, 세파덱스 레진이 충진된 컬럼을 이용하여 1차 정제하고, 순차적으로 C18 레진이 충진된 컬럼을 이용하여 2차 정제하는 방법을 통하여 단시간 내에 순도 90% 이상의 클로로필 a를 정제할 수 있는 방법에 관한 것으로, 본 발명의 분리 및 정제방법으로 정제된 클로로필 a는 항산화용 화장품 또는 항산화용 건강기능식품의 소재로 유용하게 사용될 수 있다. |
| format | Patent |
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본 발명은 미세조류로부터 고순도 클로로필 a를 분리 및 정제하는 방법에 관한 것으로, 보다 상세하게는 에탄올 및 헥산을 이용하여 미세조류로부터 클로로필 a를 추출한 후, 세파덱스 레진이 충진된 컬럼을 이용하여 1차 정제하고, 순차적으로 C18 레진이 충진된 컬럼을 이용하여 2차 정제하는 방법을 통하여 단시간 내에 순도 90% 이상의 클로로필 a를 정제할 수 있는 방법에 관한 것으로, 본 발명의 분리 및 정제방법으로 정제된 클로로필 a는 항산화용 화장품 또는 항산화용 건강기능식품의 소재로 유용하게 사용될 수 있다.</description><language>eng ; kor</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; HETEROCYCLIC COMPOUNDS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2019</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190927&DB=EPODOC&CC=KR&NR=102026235B1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20190927&DB=EPODOC&CC=KR&NR=102026235B1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHOI JIN YOUNG</creatorcontrib><creatorcontrib>CHOI, TAE O</creatorcontrib><title>a Method for isolating and purifying chlorophyll a from microalgae</title><description>The present invention relates to a method for separating and purifying high purity chlorophyll a from microalgae. More particularly, the present invention relates to a method capable of purifying chlorophyll a with a purity of 90% or more in a short time, wherein chlorophyll a is extracted from microalgae using ethanol and hexane, and then first purified using a column packed with Sephadex resin; and subjected to secondary purification using a column packed with C18 resin sequentially. Chlorophyll a purified by the separation and purification method of the present invention can be usefully used as a material for antioxidant cosmetics or functional health foods for antioxidants.
본 발명은 미세조류로부터 고순도 클로로필 a를 분리 및 정제하는 방법에 관한 것으로, 보다 상세하게는 에탄올 및 헥산을 이용하여 미세조류로부터 클로로필 a를 추출한 후, 세파덱스 레진이 충진된 컬럼을 이용하여 1차 정제하고, 순차적으로 C18 레진이 충진된 컬럼을 이용하여 2차 정제하는 방법을 통하여 단시간 내에 순도 90% 이상의 클로로필 a를 정제할 수 있는 방법에 관한 것으로, 본 발명의 분리 및 정제방법으로 정제된 클로로필 a는 항산화용 화장품 또는 항산화용 건강기능식품의 소재로 유용하게 사용될 수 있다.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>HETEROCYCLIC COMPOUNDS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2019</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHBKVPBNLcnIT1FIyy9SyCzOz0ksycxLV0jMS1EoKC3KTKsE8ZIzcvKL8gsyKnNyFBIV0orycxVyM5OL8hNz0hNTeRhY0xJzilN5oTQ3g7Kba4izh25qQX58anFBYnJqXmpJvHeQoYGRgZGZkbGpk5OhMXGqAPmRMvM</recordid><startdate>20190927</startdate><enddate>20190927</enddate><creator>CHOI JIN YOUNG</creator><creator>CHOI, TAE O</creator><scope>EVB</scope></search><sort><creationdate>20190927</creationdate><title>a Method for isolating and purifying chlorophyll a from microalgae</title><author>CHOI JIN YOUNG ; CHOI, TAE O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_KR102026235BB13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; kor</language><creationdate>2019</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>HETEROCYCLIC COMPOUNDS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CHOI JIN YOUNG</creatorcontrib><creatorcontrib>CHOI, TAE O</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CHOI JIN YOUNG</au><au>CHOI, TAE O</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>a Method for isolating and purifying chlorophyll a from microalgae</title><date>2019-09-27</date><risdate>2019</risdate><abstract>The present invention relates to a method for separating and purifying high purity chlorophyll a from microalgae. More particularly, the present invention relates to a method capable of purifying chlorophyll a with a purity of 90% or more in a short time, wherein chlorophyll a is extracted from microalgae using ethanol and hexane, and then first purified using a column packed with Sephadex resin; and subjected to secondary purification using a column packed with C18 resin sequentially. Chlorophyll a purified by the separation and purification method of the present invention can be usefully used as a material for antioxidant cosmetics or functional health foods for antioxidants.
본 발명은 미세조류로부터 고순도 클로로필 a를 분리 및 정제하는 방법에 관한 것으로, 보다 상세하게는 에탄올 및 헥산을 이용하여 미세조류로부터 클로로필 a를 추출한 후, 세파덱스 레진이 충진된 컬럼을 이용하여 1차 정제하고, 순차적으로 C18 레진이 충진된 컬럼을 이용하여 2차 정제하는 방법을 통하여 단시간 내에 순도 90% 이상의 클로로필 a를 정제할 수 있는 방법에 관한 것으로, 본 발명의 분리 및 정제방법으로 정제된 클로로필 a는 항산화용 화장품 또는 항산화용 건강기능식품의 소재로 유용하게 사용될 수 있다.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HETEROCYCLIC COMPOUNDS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY SPIRITS VINEGAR WINE |
| title | a Method for isolating and purifying chlorophyll a from microalgae |
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