a Method for isolating and purifying chlorophyll a from microalgae

The present invention relates to a method for separating and purifying high purity chlorophyll a from microalgae. More particularly, the present invention relates to a method capable of purifying chlorophyll a with a purity of 90% or more in a short time, wherein chlorophyll a is extracted from micr...

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Bibliographische Detailangaben
Hauptverfasser:	CHOI JIN YOUNG, CHOI, TAE O
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HETEROCYCLIC COMPOUNDS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method for separating and purifying high purity chlorophyll a from microalgae. More particularly, the present invention relates to a method capable of purifying chlorophyll a with a purity of 90% or more in a short time, wherein chlorophyll a is extracted from microalgae using ethanol and hexane, and then first purified using a column packed with Sephadex resin; and subjected to secondary purification using a column packed with C18 resin sequentially. Chlorophyll a purified by the separation and purification method of the present invention can be usefully used as a material for antioxidant cosmetics or functional health foods for antioxidants. 본 발명은 미세조류로부터 고순도 클로로필 a를 분리 및 정제하는 방법에 관한 것으로, 보다 상세하게는 에탄올 및 헥산을 이용하여 미세조류로부터 클로로필 a를 추출한 후, 세파덱스 레진이 충진된 컬럼을 이용하여 1차 정제하고, 순차적으로 C18 레진이 충진된 컬럼을 이용하여 2차 정제하는 방법을 통하여 단시간 내에 순도 90% 이상의 클로로필 a를 정제할 수 있는 방법에 관한 것으로, 본 발명의 분리 및 정제방법으로 정제된 클로로필 a는 항산화용 화장품 또는 항산화용 건강기능식품의 소재로 유용하게 사용될 수 있다.