FabL-h Novel FabL-h gene resistant to triclosan from soil metagenome and uses thereof

The present invention relates to a novel triclosan resistant gene derived from soil metagenomes and to uses thereof, wherein a novel triclosan resistant ENR mutant FabL-h coding gene of the present invention is expressed in various bacteria expressing Escherichia coli and an enoyl-acyl-carrier prote...

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Bibliographische Detailangaben
Hauptverfasser:	HWANG, EUL CHUL, KHAN RAEES, LEE, SEON WOO
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a novel triclosan resistant gene derived from soil metagenomes and to uses thereof, wherein a novel triclosan resistant ENR mutant FabL-h coding gene of the present invention is expressed in various bacteria expressing Escherichia coli and an enoyl-acyl-carrier protein reductase (ENR) protein to be utilized as a triclosan resistant selection marker capable of effectively selecting a triclosan resistant transforming agent, thereby being very useful in related industries. 본 발명은 토양 메타게놈 유래의 트리클로산 저항성 신규 유전자 및 이의 용도에 관한 것으로, 본 발명의 신규 트리클로산 저항성 ENR 변이체 FabL-h 코딩 유전자는 대장균뿐만 아니라 ENR(enoyl-acyl-carrier protein reductase) 단백질을 발현하는 다양한 세균에 발현시켜 트리클로산 저항성 형질전환체를 효과적으로 선별할 수 있는 트리클로산 저항성 선발 마커로 활용할 수 있어, 관련 산업에 매우 유용하다.