Method and kit for detecting Enterohaemorrhagic E. coli and Shigella dysenteriae using real-time PCR

The present invention relates to a kit and method to check enterohemorrhagic E. coli and Shigella dysenteriae by using real-time PCR, capable of detecting enterohaemorrhagic E. coli and Shigella dysenteriae in shigella by using an STX gene of shiga toxin as a primary gene marker, and also, specifica...

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Bibliographische Detailangaben
Hauptverfasser:	LEE, YANG HAN, LEE, KYUNG LYONG, CHO, YOON JUNG, KIM, HYO SOOK, LEE, DONG SUB, KIM, MIN HEE, PARK, MOON HEE, EOM, KI YOON
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a kit and method to check enterohemorrhagic E. coli and Shigella dysenteriae by using real-time PCR, capable of detecting enterohaemorrhagic E. coli and Shigella dysenteriae in shigella by using an STX gene of shiga toxin as a primary gene marker, and also, specifically classifying enterohemorrhagic E. coli and Shigella dysenteriae by using an rfpB gene of Shigella dysenteriae glycosyltransferase as a secondary gene marker. According to the present invention, specifically determining enterohemorrhagic E. coli and Shigella dysenteriae, which are not able to be classified through a serologic analysis method using shiga toxin, the present invention is capable of solving the misdiagnosis of viruses which cause diarrhea, etc. 본 발명은 시가 독소(shiga toxin)의 STX 유전자를 1차 유전자 마커로 사용하여, 병원성 대장균 중 장출혈성 대장균과, 시겔라(속의 세균 중 시겔라 디젠테리를 검출하고, 시겔라 디젠테리 글리코실 전달효소(glycosyl transferase)의 rfpB 유전자를 2차 유전자 마커로 사용하여 장출혈성 대장균과 시겔라 디젠테리를 특이적으로 구분할 수 있는 리얼타임 PCR을 이용한 장출혈성 대장균 및 시겔라 디젠테리의 신속 검사 키트 및 방법을 제공한다. 본 발명에 따르면, 시가 독소를 이용한 혈청분석법으로는 구분할 수 없는 장출혈성 대장균(Enterohaemorrhagic)과 시겔라 디젠테리()를 특이적으로 판별하여 설사 증세 등을 일으키는 원인균을 잘못 진단하는 문제점을 해결할 수 있다.