RNA METHOD AND APPARATUS FOR ULTRASENSITIVE QUANTIFICATION OF MICRORNA

The present invention relates to a method for detecting unlabeled microRNA at supersensitivity using a hybrid binding domain (HBD)-immobilized probe which is used in atomic force microscopy and specifically binds to double strands of RNA and DNA. The present invention further relates to a quantitati...

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Bibliographische Detailangaben
Hauptverfasser:	KIM, JOUNG HUN, KOO, HYUN SEO, PARK, JOON WON, KIM, YOUNG KYU, LEE, YOON HEE
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBEMICROSCOPY [SPM] BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SCANNING-PROBE TECHNIQUES OR APPARATUS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method for detecting unlabeled microRNA at supersensitivity using a hybrid binding domain (HBD)-immobilized probe which is used in atomic force microscopy and specifically binds to double strands of RNA and DNA. The present invention further relates to a quantitative analysis apparatus which includes unlabeled microRNA and the HBD-immobilized probe for atomic force microscopy. 본 발명은 DNA와 RNA의 이중가닥에 특이적으로 결합하는 하이브리드 결합 도메인 (hybrid binding domain, HBD)이 고정된 원자간력현미경의 탐침을 이용하여 무표지의 마이크로RNA를 초고민감도로 검출하는 방법 및 상기 하이브리드 결합도메인이 고정된 원자간력현미경의 탐침 및 무표지의 마이크로 RNA를 포함하는 정량분석장치에 관한 것이다.