KR100262867B

KR100262867B

PURPOSE: Provided is a method for manufacturing human granulocyte colony stimulating factor in a high yield by increasing the ratio of A/T at N-terminal of the rHuG-CSF. CONSTITUTION: Human granulocyte colony stimulating factor (HuG-CSF) is manufactured from E. coli by using plasmid pT7GCSF which is...

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Bibliographische Detailangaben
Hauptverfasser: OH, MYONG-SEOK, KIM, HYON-SOO, KO, HYONG-KON, LEE, HYON-SOO, HA, SEOK-HOON, JUNE, KEUN-TAEK, MOK-HEON
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PURPOSE: Provided is a method for manufacturing human granulocyte colony stimulating factor in a high yield by increasing the ratio of A/T at N-terminal of the rHuG-CSF. CONSTITUTION: Human granulocyte colony stimulating factor (HuG-CSF) is manufactured from E. coli by using plasmid pT7GCSF which is included in a segment of hG-CSF gene coding HuG-CSF and is controlled by promoter T7, wherein the base sequence at N-terminal of a gene coding HuG-CSF is 5'-ATGACACCTTTAGACCT-3'. While unnecessary amino acids are not inserted into N-terminal, the increase in the ratio of A/T on N-terminal of the rHuG-CSF allows production of a massive amount of HuG-CSF.