CONTAINMENT CUVETTE FOR PCR AND METHOD OF USE

There are disclosed a cuvette (10, 100, 100C, 100D, 100E) and a method of use which prevent nucleic acid amplified by PCR technology from being released to the atmosphere, while still proceeding to a detection step to determine whether or not the nucleic acid is present. Detection reagents are eithe...

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Hauptverfasser:	SCHNIPELSKY, PAUL N, SEABERG, LEONARD J, FINDLAY, JOHN B, DONISHI, WILLIAM H, HINCKLEY, CHARLES C, WELLMANN, JEFFREY A
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	There are disclosed a cuvette (10, 100, 100C, 100D, 100E) and a method of use which prevent nucleic acid amplified by PCR technology from being released to the atmosphere, while still proceeding to a detection step to determine whether or not the nucleic acid is present. Detection reagents are either pre-incorporated into compartments (30, 30A, 30B, 32, 32A, 32B, 34, 34A, 34B, 36, 36A, 36B, 38, 38A, 38B, 40, 40A, 40B) and (132, 132A, 132B, 132C, 132D, 132E, 134, 134A, 134B, 134C, 134D, 134E, 136, 136A, 136B, 136C, 136D, 136E, 138, 138A, 138B, 138C, 138D, 138E, 139, 139A, 139B, 139C, 139D, 139E) in the cuvette or added after amplification. In the latter case, a check valve prevents amplified nucleic acid from being released. Transfer of liquids between compartments is achieved via the use of flexible compartment walls and an external pressure source (60), or via pistons (113, 113C, 113D, 115, 115C, 115D, 184, 184C, 184E, 260) that are part of the cuvette and operate on the compartments as a piston within a piston chamber.