COSMETIC COMPOSITION CONTAINING BIO-SURFACTANT

After culturing Acinetobacter calcoaceticus CL on the phosphate buffer culture media containing ethanol and urea at 30 deg.C for 2 days, ethylenediamine tetraacetate is added. Then it is stirred at 65 deg.C, cooled, and centrifuged to obtain the supernatant. The surfactant is purified through saltin...

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Bibliographische Detailangaben
Hauptverfasser: KIM, EUI, HWANG, KYUNG-AH
Format: Patent
Sprache:eng ; kor
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Zusammenfassung:After culturing Acinetobacter calcoaceticus CL on the phosphate buffer culture media containing ethanol and urea at 30 deg.C for 2 days, ethylenediamine tetraacetate is added. Then it is stirred at 65 deg.C, cooled, and centrifuged to obtain the supernatant. The surfactant is purified through salting-out and dialysis by adding ammonium sulfate. Then through ultrafiltration and freeze-drying, the bio-surfactant powder is obtained. The cosmetic formulation with excellent stability, moisturizing, emulsifying, and softening effects is prepared by adding this bio-surfactant at 0.01-10.0% w/w. 본 발명은 자연계로부터 순수 분리한 계면활성제 생산 균주, 아시네토박터 칼로아세티쿠스 CL(Acinetobacter calcoaceticus CL ; KCTC 0081BP)로부터 생산된 생물계면활성제를 화장료에 함유시킴으로써 제조된, 피부보습 및 유연효과가 탁월하고 피부안전성 및 제품으로서의 안정성이 뛰어난 화장료 조성물에 관한 것이다.