PRODUCTION OF FAT AND OIL

PURPOSE:To obtain a fat and oil suitable for food, cosmetic industries, etc., in high yield without oxidation or coloration, by reacting an enzyme-lipid complex which is a nonaqueous highly active enzyme with a fatty acid and fatty acid monoglyceride in a nonaqueous organic solvent. CONSTITUTION:(A) An enzyme, such as lipase, in an amount of 1mg is dissolved in 0.02-20ml buffer solution at, e.g. pH 5.0-7.0, and (B) a solution prepared by dissolving, e.g. 10mg lipid (e.g. monogalactosyldiglyceride) in a hydrophilic organic solvent, such as methanol, is dripped thereon, stirred and dispersed while cooling to provide an enzyme-lipid complex of the nonaqueous highly active enzyme, which is subsequently dispersed in (C) an organic solvent, such as hexane. (D) A 4-24C fatty acid (e.g. lauric acid) and monoglyceride are then preferably added and reacted to afford the aimed fat and oil.