MICROINJECTION INTO CELL OR SUCTION FROM INDIVIDUAL CELL OR METHOD AND WORKING STATION SUCKING ALL CELLS FROM CELL INCUBATION

PURPOSE: To quickly and surely conduct work by converting picture coordinates into the object coordinates of a cell carrier positioning device and positioning capillary tubes to the object coordinates calculated relatively to each cell. CONSTITUTION: The stage 5 of a microscope 1 is moved in the x-,...

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Bibliographische Detailangaben
Hauptverfasser: FUUBERUTO NATSUSE, BUARUTAA GAISU, ARUPUREHITO KETORAA, FUORUKAA BUIRUKE, BUIRUHERUMU ANZORUGE
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:PURPOSE: To quickly and surely conduct work by converting picture coordinates into the object coordinates of a cell carrier positioning device and positioning capillary tubes to the object coordinates calculated relatively to each cell. CONSTITUTION: The stage 5 of a microscope 1 is moved in the x-, y-, and z- directions by means of the motor control section 17 of a computer 11. Capillary tubes 4 are connected to an injecting device 9 through a pressure resistant tube 8, fixed to a certain moving device, and obliquely gets in a sample container on the stage 5. A graphic card 13 is connected to a monitor 10 containing a mixing stage and a mark generated from the card 13 is superimposed upon the video signal of a TV camera 2. A marker is sent to the picture of the monitor 10 by using a graphic tablet 12. The computer 11 is connected to the injecting device 9 of the capillary tube 4 through an interface 15 and controls the injecting time and injecting amount of the device 9. The injecting pressure of the device 9, in addition, is also controlled by time in accordance with the movement of the tube 4. Therefore, work can be carried out quickly and surely.