RECOMBINANT PLASMID AND RECOMBINANT CONTAINING HUMAN LYSOZYME GENE
PURPOSE:To efficiently produce human lysozyme, by transforming an yeast with a recombinant plasmid holding a human lysozyme gene linked to a secretory leader sequence and processing signal sequence. CONSTITUTION:A synthetic oligonucleotide, expressed by the formula linked to alpha-factor gene at the...
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| creator | YASUKUI HIDEO AKIMARU HITOAKI GOMI HIDEYUKI |
| description | PURPOSE:To efficiently produce human lysozyme, by transforming an yeast with a recombinant plasmid holding a human lysozyme gene linked to a secretory leader sequence and processing signal sequence. CONSTITUTION:A synthetic oligonucleotide, expressed by the formula linked to alpha-factor gene at the HindIII terminal and to human lysozyme gene at the TaqI terminal is prepared. A DNA fragment containing a synthetic human lysozyme and leader processing signal of an alpha-factor is linked to the above- mentioned nucleotide by treatment with T4 ligase to give a recombinant plasmid pLY26. A DNA fragment containing a selection marker TRP1 and a replication starting point ARS1 is linked to the above-mentioned plasmid to provide a plasmid pLY27, which is then linked to a DNA fragment containing a 2mum plasmid replication starting point to construct a recombinant plasmid pLY28. An yeast NA87-11A strain can be transformed by the above-mentioned plasmid to produce the aimed human lysozyme. |
| format | Patent |
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CONSTITUTION:A synthetic oligonucleotide, expressed by the formula linked to alpha-factor gene at the HindIII terminal and to human lysozyme gene at the TaqI terminal is prepared. A DNA fragment containing a synthetic human lysozyme and leader processing signal of an alpha-factor is linked to the above- mentioned nucleotide by treatment with T4 ligase to give a recombinant plasmid pLY26. A DNA fragment containing a selection marker TRP1 and a replication starting point ARS1 is linked to the above-mentioned plasmid to provide a plasmid pLY27, which is then linked to a DNA fragment containing a 2mum plasmid replication starting point to construct a recombinant plasmid pLY28. An yeast NA87-11A strain can be transformed by the above-mentioned plasmid to produce the aimed human lysozyme.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | RECOMBINANT PLASMID AND RECOMBINANT CONTAINING HUMAN LYSOZYME GENE |
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