MICROBIAL PRODUCTION OF HUMAN LYSOZYME

PURPOSE:To make is possible to mass produce human lysozyme protein, by transforming a bacgerium with a plasmid integrated with a specific DNA sequence. CONSTITUTION:A human lysozyme gene having DNA fragment shown by the formula at the 5' end is integrated into a plasmid and a host bacterium is transformed with the prepared integrated plasmid. It is found that translation activity is extremely raised when an initiation codon and Shine-Dalgarno sequence (liposome bond site) are made into a secondary structure not to form hydrogen bond with a complementary base. Further it is found that human lysozyme is developed efficiently and in large amounts when a small fragment between EcoRI-SalI site of pUC plasmid having an extremely large number of copies in cell because of occurrence of variation in the control system of replication starting site is replaced with EcoRI-SalI fragment of pHLHLY-2 containing lambdaPL promoter having powerful transcription activity capable of introducing transcription by temperature and human lysozyme.