PRODUCTION OF MENAQUINONE-4 BY FERMENTATION

PRODUCTION OF MENAQUINONE-4 BY FERMENTATION

PURPOSE:To produce menaquinone-4 participating in the coagulation of blood and control of calcium in living body, in high efficiency, by culturing a menaquinone-4-producing microorganism in a medium containing lactose as a carbon source. CONSTITUTION:Corynebacterium liquefaciens ATCC 14929, etc., ar...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: ARAI TAKEMITSU, TOMOHIRO SUSUMU, ENDO SHIZUKO
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:PURPOSE:To produce menaquinone-4 participating in the coagulation of blood and control of calcium in living body, in high efficiency, by culturing a menaquinone-4-producing microorganism in a medium containing lactose as a carbon source. CONSTITUTION:Corynebacterium liquefaciens ATCC 14929, etc., are cultured in a medium containing 5-200g/l, preferably 20-180g/l of glucose as a carbon source in addition to nitrogen source, inorganic salts, minor element, etc., at 20-40 deg.C, preferably 25-35 deg.C and pH 5-9, preferably about 7 for 3-7 days under aerobic condition and the objective menaquinone-4 is separated from the cultured product using a solvent, etc.