HEPATIC CELL GROWTH FACTOR

HEPATIC CELL GROWTH FACTOR

NEW MATERIAL:A hepatic cell growth factor [estimated molecular weight by SDS-PAGE (under non-reducing condition), 76,000-92,000; active to enable growth of hepatic cell; losing the above activity by the heat-treatment at 80 deg.C for 10min; losing the above activity by trypsin digestion and chymotry...

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Hauptverfasser: TSUBOUCHI HIROHITO, NAKAYAMA HIROYUKI, TAKAHASHI KOZO, DAIKUHARA YASUSHI, AIDA EIICHI, HASHIMOTO SHUJI, HIRONO SHIYUUICHI
Format: Patent
Sprache:eng
Schlagworte:
CHEMISTRY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
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Zusammenfassung:NEW MATERIAL:A hepatic cell growth factor [estimated molecular weight by SDS-PAGE (under non-reducing condition), 76,000-92,000; active to enable growth of hepatic cell; losing the above activity by the heat-treatment at 80 deg.C for 10min; losing the above activity by trypsin digestion and chymotrypsin digestion; having strong affinity to heparin]. USE:Remedy for hepatic diseases such as acute hepatitis, chronic hepatitis, hepatocirrhosis, etc., and post-operative remedy for hepatectomy. Antigen for establishing immunological diagnosis of various diseases. PREPARATION:Human blood, especially blood (plasma) of patient of fulminant hepatitis is heated at 56 deg.C for about 15min, fractionated with ammonium sulfate and a fraction precipitated at an ammonium sulfate concentration of about 1.1-2.1M is collected to obtain the active component. The obtained component can be purified by gel-filtration using a carrier, ion exchange chromatography using an anion exchange material such as DEAE, etc.